Development and validation of RP-HPLC method for simultaneous estimation of rosuvastatin and bisoprolol fumarate in bulk and formulations

A simple, accurate and precise RP-HPLC method was developed for simultaneous estimation of Rosuvastatin Bisoprolol fumarate in bulk formulation. Separation achieved using C18 column Methanol: Phosphate buffer (pH 3.5) at a ratio 45:55% V/V, flow rate maintained 1.0 ml. min 245 nm used as determination wavelength. System suitability parameters shows values within specified limits. Developed validated per ICH guidelines all the were found to be with limits regulatory guidelines. Linearity 4-20 8-40 mcg/ml respectively. Results accuracy 98-102% which method.